Construct: shRNA TRCN0000161340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020431.1-1625s1c1
DNA Barcode:
GCTACATCTTTCTGGTGTTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM63C (57156)
Gene Description:
transmembrane protein 63C
Transcript:
RefSeq NM_020431.1 (NON-CURRENT)
Match location:
Position 1625 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000161340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACATCTTTCTGGTGTTCA
Hairpin Sequence:
5'-CCGG-GCTACATCTTTCTGGTGTTCA-CTCGAG-TGAACACCAGAAAGATGTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACATCTTTCTGGTGTTCACTCGAGTGAACACCAGAAAGATGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACATCTTTCTGGTGTTCACTCGAGTGAACACCAGAAAGATGTAGC-3'

Other clones with same target sequence:

(none)