Construct: shRNA TRCN0000161371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153215.1-1067s1c1
DNA Barcode:
GCTGACCGTTCTGTTTAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LSMEM2 (132228)
Gene Description:
leucine rich single-pass membrane protein 2
Transcript:
RefSeq NM_153215.1 (NON-CURRENT)
Match location:
Position 1067 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000161371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACCGTTCTGTTTAATTA
Hairpin Sequence:
5'-CCGG-GCTGACCGTTCTGTTTAATTA-CTCGAG-TAATTAAACAGAACGGTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACCGTTCTGTTTAATTACTCGAGTAATTAAACAGAACGGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACCGTTCTGTTTAATTACTCGAGTAATTAAACAGAACGGTCAGC-3'

Other clones with same target sequence:

(none)