Construct: shRNA TRCN0000161472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018312.2-2384s1c1
DNA Barcode:
GCAGAAAGTACAGACAAGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP6R3 (55291)
Gene Description:
protein phosphatase 6 regulatory subunit 3
Transcript:
RefSeq NM_018312.2 (NON-CURRENT)
Match location:
Position 2384 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000161472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAAGTACAGACAAGGTA
Hairpin Sequence:
5'-CCGG-GCAGAAAGTACAGACAAGGTA-CTCGAG-TACCTTGTCTGTACTTTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAAGTACAGACAAGGTACTCGAGTACCTTGTCTGTACTTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAAGTACAGACAAGGTACTCGAGTACCTTGTCTGTACTTTCTGC-3'

Other clones with same target sequence:

(none)