Construct: shRNA TRCN0000161711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152540.3-2770s1c1
DNA Barcode:
GCCATAACAAAGGACTGCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCFD2 (152579)
Gene Description:
sec1 family domain containing 2
Transcript:
RefSeq NM_152540.3 (NON-CURRENT)
Match location:
Position 2770 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000161711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATAACAAAGGACTGCATT
Hairpin Sequence:
5'-CCGG-GCCATAACAAAGGACTGCATT-CTCGAG-AATGCAGTCCTTTGTTATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATAACAAAGGACTGCATTCTCGAGAATGCAGTCCTTTGTTATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATAACAAAGGACTGCATTCTCGAGAATGCAGTCCTTTGTTATGGC-3'

Other clones with same target sequence:

(none)