Construct: shRNA TRCN0000161885

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007005.3-3356s1c1
DNA Barcode:
GCTGTCTACTTGGAAGAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TLE4 (7091)
Gene Description:
TLE family member 4, transcriptional corepressor
Transcript:
RefSeq NM_007005.3 (NON-CURRENT)
Match location:
Position 3356 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000161885)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCTACTTGGAAGAACAT
Hairpin Sequence:
5'-CCGG-GCTGTCTACTTGGAAGAACAT-CTCGAG-ATGTTCTTCCAAGTAGACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCTACTTGGAAGAACATCTCGAGATGTTCTTCCAAGTAGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCTACTTGGAAGAACATCTCGAGATGTTCTTCCAAGTAGACAGC-3'

Other clones with same target sequence:

  1. TRCN0000285323