Construct: shRNA TRCN0000162020

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012449.2-1017s1c1
DNA Barcode:
GCCTGGAATAAGTGGATAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STEAP1 (26872)
Gene Description:
STEAP family member 1
Transcript:
RefSeq NM_012449.2 (NON-CURRENT)
Match location:
Position 1017 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162020)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGAATAAGTGGATAGAT
Hairpin Sequence:
5'-CCGG-GCCTGGAATAAGTGGATAGAT-CTCGAG-ATCTATCCACTTATTCCAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGAATAAGTGGATAGATCTCGAGATCTATCCACTTATTCCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGAATAAGTGGATAGATCTCGAGATCTATCCACTTATTCCAGGC-3'

Other clones with same target sequence:

(none)