Construct: shRNA TRCN0000162171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004440.1-793s1c1
DNA Barcode:
CAGTTGAATTGGAGAGTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAFA3 (284467)
Gene Description:
TAFA chemokine like family member 3
Transcript:
RefSeq NM_001004440.1 (CURRENT)
Match location:
Position 793 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTGAATTGGAGAGTTGAT
Hairpin Sequence:
5'-CCGG-CAGTTGAATTGGAGAGTTGAT-CTCGAG-ATCAACTCTCCAATTCAACTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTGAATTGGAGAGTTGATCTCGAGATCAACTCTCCAATTCAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTGAATTGGAGAGTTGATCTCGAGATCAACTCTCCAATTCAACTG-3'

Other clones with same target sequence:

(none)