Construct: shRNA TRCN0000162176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003763.2-968s1c1
DNA Barcode:
CGTTGAACAGTCCTGTATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STX16 (8675)
Gene Description:
syntaxin 16
Transcript:
RefSeq NM_003763.2 (NON-CURRENT)
Match location:
Position 968 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTGAACAGTCCTGTATCAA
Hairpin Sequence:
5'-CCGG-CGTTGAACAGTCCTGTATCAA-CTCGAG-TTGATACAGGACTGTTCAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTGAACAGTCCTGTATCAACTCGAGTTGATACAGGACTGTTCAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTGAACAGTCCTGTATCAACTCGAGTTGATACAGGACTGTTCAACG-3'

Other clones with same target sequence:

(none)