Construct: shRNA TRCN0000162208

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001008737.1-1702s1c1
DNA Barcode:
CGATCTACTTGCTACTTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC401052 (401052)
Gene Description:
uncharacterized LOC401052
Transcript:
RefSeq NM_001008737.1 (NON-CURRENT)
Match location:
Position 1702 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162208)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATCTACTTGCTACTTCTAT
Hairpin Sequence:
5'-CCGG-CGATCTACTTGCTACTTCTAT-CTCGAG-ATAGAAGTAGCAAGTAGATCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATCTACTTGCTACTTCTATCTCGAGATAGAAGTAGCAAGTAGATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATCTACTTGCTACTTCTATCTCGAGATAGAAGTAGCAAGTAGATCG-3'

Other clones with same target sequence:

(none)