Construct: shRNA TRCN0000162272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018109.2-2095s1c1
DNA Barcode:
CGTGGACAACATAGCAAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTPAP (55149)
Gene Description:
mitochondrial poly(A) polymerase
Transcript:
RefSeq NM_018109.2 (NON-CURRENT)
Match location:
Position 2095 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGACAACATAGCAAGATT
Hairpin Sequence:
5'-CCGG-CGTGGACAACATAGCAAGATT-CTCGAG-AATCTTGCTATGTTGTCCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGACAACATAGCAAGATTCTCGAGAATCTTGCTATGTTGTCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGACAACATAGCAAGATTCTCGAGAATCTTGCTATGTTGTCCACG-3'

Other clones with same target sequence:

(none)