Construct: shRNA TRCN0000162333

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024586.3-1826s1c1
DNA Barcode:
CACAGAATTACTGCCGAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OSBPL9 (114883)
Gene Description:
oxysterol binding protein like 9
Transcript:
RefSeq NM_024586.3 (NON-CURRENT)
Match location:
Position 1826 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162333)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAGAATTACTGCCGAGATT
Hairpin Sequence:
5'-CCGG-CACAGAATTACTGCCGAGATT-CTCGAG-AATCTCGGCAGTAATTCTGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAGAATTACTGCCGAGATTCTCGAGAATCTCGGCAGTAATTCTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAGAATTACTGCCGAGATTCTCGAGAATCTCGGCAGTAATTCTGTG-3'

Other clones with same target sequence:

(none)