Construct: shRNA TRCN0000162433

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152540.3-1838s1c1
DNA Barcode:
CCAGGCATCTTATAAGCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCFD2 (152579)
Gene Description:
sec1 family domain containing 2
Transcript:
RefSeq NM_152540.3 (NON-CURRENT)
Match location:
Position 1838 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162433)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCATCTTATAAGCCATT
Hairpin Sequence:
5'-CCGG-CCAGGCATCTTATAAGCCATT-CTCGAG-AATGGCTTATAAGATGCCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCATCTTATAAGCCATTCTCGAGAATGGCTTATAAGATGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCATCTTATAAGCCATTCTCGAGAATGGCTTATAAGATGCCTGG-3'

Other clones with same target sequence:

(none)