Construct: shRNA TRCN0000162496

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024120.2-598s1c1
DNA Barcode:
CACTCTATGAACTTCGGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDUFAF5 (79133)
Gene Description:
NADH:ubiquinone oxidoreductase complex assembly factor 5
Transcript:
RefSeq NM_024120.2 (NON-CURRENT)
Match location:
Position 598 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162496)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTCTATGAACTTCGGTGTT
Hairpin Sequence:
5'-CCGG-CACTCTATGAACTTCGGTGTT-CTCGAG-AACACCGAAGTTCATAGAGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTCTATGAACTTCGGTGTTCTCGAGAACACCGAAGTTCATAGAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTCTATGAACTTCGGTGTTCTCGAGAACACCGAAGTTCATAGAGTG-3'

Other clones with same target sequence:

(none)