Construct: shRNA TRCN0000162509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015916.3-1290s1c1
DNA Barcode:
CTCAACAAGGATGATGAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CALHM2 (51063)
Gene Description:
calcium homeostasis modulator family member 2
Transcript:
RefSeq NM_015916.3 (NON-CURRENT)
Match location:
Position 1290 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAACAAGGATGATGAGGAA
Hairpin Sequence:
5'-CCGG-CTCAACAAGGATGATGAGGAA-CTCGAG-TTCCTCATCATCCTTGTTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAACAAGGATGATGAGGAACTCGAGTTCCTCATCATCCTTGTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAACAAGGATGATGAGGAACTCGAGTTCCTCATCATCCTTGTTGAG-3'

Other clones with same target sequence:

(none)