Construct: shRNA TRCN0000162623

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015442.1-2007s1c1
DNA Barcode:
CGACAGAATATCTGATGCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNOT10 (25904)
Gene Description:
CCR4-NOT transcription complex subunit 10
Transcript:
RefSeq NM_015442.1 (NON-CURRENT)
Match location:
Position 2007 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162623)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACAGAATATCTGATGCCAT
Hairpin Sequence:
5'-CCGG-CGACAGAATATCTGATGCCAT-CTCGAG-ATGGCATCAGATATTCTGTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACAGAATATCTGATGCCATCTCGAGATGGCATCAGATATTCTGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACAGAATATCTGATGCCATCTCGAGATGGCATCAGATATTCTGTCG-3'

Other clones with same target sequence:

(none)