Construct: shRNA TRCN0000162634

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000854.2-115s1c1
DNA Barcode:
CTACATCTTCGCCAAGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSTT2 (2953)
Gene Description:
glutathione S-transferase theta 2 (gene/pseudogene)
Transcript:
RefSeq NM_000854.2 (NON-CURRENT)
Match location:
Position 115 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162634)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACATCTTCGCCAAGAAGAA
Hairpin Sequence:
5'-CCGG-CTACATCTTCGCCAAGAAGAA-CTCGAG-TTCTTCTTGGCGAAGATGTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACATCTTCGCCAAGAAGAACTCGAGTTCTTCTTGGCGAAGATGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACATCTTCGCCAAGAAGAACTCGAGTTCTTCTTGGCGAAGATGTAG-3'

Other clones with same target sequence:

(none)