Construct: shRNA TRCN0000162672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018338.2-2429s1c1
DNA Barcode:
CCTATTGATGTCCGTTATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFAP44 (55779)
Gene Description:
cilia and flagella associated protein 44
Transcript:
RefSeq NM_018338.2 (NON-CURRENT)
Match location:
Position 2429 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATTGATGTCCGTTATCTT
Hairpin Sequence:
5'-CCGG-CCTATTGATGTCCGTTATCTT-CTCGAG-AAGATAACGGACATCAATAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATTGATGTCCGTTATCTTCTCGAGAAGATAACGGACATCAATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATTGATGTCCGTTATCTTCTCGAGAAGATAACGGACATCAATAGG-3'

Other clones with same target sequence:

(none)