Construct: shRNA TRCN0000162682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177401.4-1319s1c1
DNA Barcode:
CATCGAGAGCTTTGTGAATCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MIDN (90007)
Gene Description:
midnolin
Transcript:
RefSeq NM_177401.4 (CURRENT)
Match location:
Position 1319 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGAGAGCTTTGTGAATCA
Hairpin Sequence:
5'-CCGG-CATCGAGAGCTTTGTGAATCA-CTCGAG-TGATTCACAAAGCTCTCGATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGAGAGCTTTGTGAATCACTCGAGTGATTCACAAAGCTCTCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGAGAGCTTTGTGAATCACTCGAGTGATTCACAAAGCTCTCGATG-3'

Other clones with same target sequence:

(none)