Construct: shRNA TRCN0000162741

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020750.1-3387s1c1
DNA Barcode:
CCTGGAGATAAGAGCTGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPO5 (57510)
Gene Description:
exportin 5
Transcript:
RefSeq NM_020750.1 (NON-CURRENT)
Match location:
Position 3387 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162741)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAGATAAGAGCTGTAAT
Hairpin Sequence:
5'-CCGG-CCTGGAGATAAGAGCTGTAAT-CTCGAG-ATTACAGCTCTTATCTCCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAGATAAGAGCTGTAATCTCGAGATTACAGCTCTTATCTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAGATAAGAGCTGTAATCTCGAGATTACAGCTCTTATCTCCAGG-3'

Other clones with same target sequence:

(none)