Construct: shRNA TRCN0000162792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014657.1-3727s1c1
DNA Barcode:
CCCTTAGCCTACCATCTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTI1 (9675)
Gene Description:
TELO2 interacting protein 1
Transcript:
RefSeq NM_014657.1 (NON-CURRENT)
Match location:
Position 3727 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTAGCCTACCATCTCTAT
Hairpin Sequence:
5'-CCGG-CCCTTAGCCTACCATCTCTAT-CTCGAG-ATAGAGATGGTAGGCTAAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTAGCCTACCATCTCTATCTCGAGATAGAGATGGTAGGCTAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTAGCCTACCATCTCTATCTCGAGATAGAGATGGTAGGCTAAGGG-3'

Other clones with same target sequence:

(none)