Construct: shRNA TRCN0000162794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015122.1-2279s1c1
DNA Barcode:
CACAACCGCTATTGAGCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FCHO1 (23149)
Gene Description:
FCH and mu domain containing endocytic adaptor 1
Transcript:
RefSeq NM_015122.1 (NON-CURRENT)
Match location:
Position 2279 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAACCGCTATTGAGCACTT
Hairpin Sequence:
5'-CCGG-CACAACCGCTATTGAGCACTT-CTCGAG-AAGTGCTCAATAGCGGTTGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAACCGCTATTGAGCACTTCTCGAGAAGTGCTCAATAGCGGTTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAACCGCTATTGAGCACTTCTCGAGAAGTGCTCAATAGCGGTTGTG-3'

Other clones with same target sequence:

(none)