Construct: shRNA TRCN0000162874

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005751.1-2941s1c1
DNA Barcode:
GATCGGGAAGATACAAGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WASHC2A (387680)
Gene Description:
WASH complex subunit 2A
Transcript:
RefSeq NM_001005751.1 (NON-CURRENT)
Match location:
Position 2941 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162874)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCGGGAAGATACAAGCAAA
Hairpin Sequence:
5'-CCGG-GATCGGGAAGATACAAGCAAA-CTCGAG-TTTGCTTGTATCTTCCCGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCGGGAAGATACAAGCAAACTCGAGTTTGCTTGTATCTTCCCGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCGGGAAGATACAAGCAAACTCGAGTTTGCTTGTATCTTCCCGATC-3'

Other clones with same target sequence:

  1. TRCN0000344332