Construct: shRNA TRCN0000162902

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001014373.1-1785s1c1
DNA Barcode:
GATCTGTGAATACAGTGCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C19orf31 (404664)
Gene Description:
chromosome 19 open reading frame 31
Transcript:
RefSeq NM_001014373.1 (NON-CURRENT)
Match location:
Position 1785 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162902)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCTGTGAATACAGTGCCTT
Hairpin Sequence:
5'-CCGG-GATCTGTGAATACAGTGCCTT-CTCGAG-AAGGCACTGTATTCACAGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCTGTGAATACAGTGCCTTCTCGAGAAGGCACTGTATTCACAGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCTGTGAATACAGTGCCTTCTCGAGAAGGCACTGTATTCACAGATC-3'

Other clones with same target sequence:

(none)