Construct: shRNA TRCN0000162923

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178275.3-1312s1c1
DNA Barcode:
GAAGCCGGTGATAGTGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGFN1 (91156)
Gene Description:
immunoglobulin like and fibronectin type III domain containing 1
Transcript:
RefSeq NM_178275.3 (NON-CURRENT)
Match location:
Position 1312 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000162923)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCCGGTGATAGTGAAGAT
Hairpin Sequence:
5'-CCGG-GAAGCCGGTGATAGTGAAGAT-CTCGAG-ATCTTCACTATCACCGGCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCCGGTGATAGTGAAGATCTCGAGATCTTCACTATCACCGGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCCGGTGATAGTGAAGATCTCGAGATCTTCACTATCACCGGCTTC-3'

Other clones with same target sequence:

(none)