Construct: shRNA TRCN0000163066

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021645.2-3883s1c1
DNA Barcode:
CCATTGCAGATCAGAGCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UTP14C (9724)
Gene Description:
UTP14C small subunit processome component
Transcript:
RefSeq NM_021645.2 (NON-CURRENT)
Match location:
Position 3883 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163066)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGCAGATCAGAGCCTTT
Hairpin Sequence:
5'-CCGG-CCATTGCAGATCAGAGCCTTT-CTCGAG-AAAGGCTCTGATCTGCAATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGCAGATCAGAGCCTTTCTCGAGAAAGGCTCTGATCTGCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGCAGATCAGAGCCTTTCTCGAGAAAGGCTCTGATCTGCAATGG-3'

Other clones with same target sequence:

(none)