Construct: shRNA TRCN0000163120

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004711.3-2795s1c1
DNA Barcode:
GCTGGTTGTTGCTGACTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYNGR1 (9145)
Gene Description:
synaptogyrin 1
Transcript:
RefSeq NM_004711.3 (NON-CURRENT)
Match location:
Position 2795 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163120)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTTGTTGCTGACTTCAT
Hairpin Sequence:
5'-CCGG-GCTGGTTGTTGCTGACTTCAT-CTCGAG-ATGAAGTCAGCAACAACCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTTGTTGCTGACTTCATCTCGAGATGAAGTCAGCAACAACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTTGTTGCTGACTTCATCTCGAGATGAAGTCAGCAACAACCAGC-3'

Other clones with same target sequence:

(none)