Construct: shRNA TRCN0000163134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173669.1-592s1c1
DNA Barcode:
GAAGATGCGTTCACTTTGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BASP1-AS1 (285696)
Gene Description:
BASP1 antisense RNA 1
Transcript:
RefSeq NM_173669.1 (NON-CURRENT)
Match location:
Position 592 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATGCGTTCACTTTGCTT
Hairpin Sequence:
5'-CCGG-GAAGATGCGTTCACTTTGCTT-CTCGAG-AAGCAAAGTGAACGCATCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATGCGTTCACTTTGCTTCTCGAGAAGCAAAGTGAACGCATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATGCGTTCACTTTGCTTCTCGAGAAGCAAAGTGAACGCATCTTC-3'

Other clones with same target sequence:

(none)