Construct: shRNA TRCN0000163139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033723.1-1056s1c1
DNA Barcode:
GAACAGAAACTCCTTGTGCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF704 (619279)
Gene Description:
zinc finger protein 704
Transcript:
RefSeq NM_001033723.1 (NON-CURRENT)
Match location:
Position 1056 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAGAAACTCCTTGTGCCA
Hairpin Sequence:
5'-CCGG-GAACAGAAACTCCTTGTGCCA-CTCGAG-TGGCACAAGGAGTTTCTGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAGAAACTCCTTGTGCCACTCGAGTGGCACAAGGAGTTTCTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAGAAACTCCTTGTGCCACTCGAGTGGCACAAGGAGTTTCTGTTC-3'

Other clones with same target sequence:

(none)