Construct: shRNA TRCN0000163141

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001649.2-590s1c1
DNA Barcode:
GCACCTTAGATCACTTCAGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SHROOM2 (357)
Gene Description:
shroom family member 2
Transcript:
RefSeq NM_001649.2 (NON-CURRENT)
Match location:
Position 590 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163141)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTTAGATCACTTCAGCT
Hairpin Sequence:
5'-CCGG-GCACCTTAGATCACTTCAGCT-CTCGAG-AGCTGAAGTGATCTAAGGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTTAGATCACTTCAGCTCTCGAGAGCTGAAGTGATCTAAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTTAGATCACTTCAGCTCTCGAGAGCTGAAGTGATCTAAGGTGC-3'

Other clones with same target sequence:

(none)