Construct: shRNA TRCN0000163188
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_178275.3-3584s1c1
- DNA Barcode:
- GCTCCAAGCTAAGTCACTCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IGFN1 (91156)
- Gene Description:
- immunoglobulin like and fibronectin type III domain containing 1
- Transcript:
- RefSeq NM_178275.3 (NON-CURRENT)
- Match location:
- Position 3584 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000163188)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCCAAGCTAAGTCACTCAA
- Hairpin Sequence:
-
5'-CCGG-GCTCCAAGCTAAGTCACTCAA-CTCGAG-TTGAGTGACTTAGCTTGGAGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCCAAGCTAAGTCACTCAACTCGAGTTGAGTGACTTAGCTTGGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCCAAGCTAAGTCACTCAACTCGAGTTGAGTGACTTAGCTTGGAGC-3'
Other clones with same target sequence:
(none)
