Construct: shRNA TRCN0000163240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032314.2-663s1c1
DNA Barcode:
GATCCGGAATGTCACACACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COQ5 (84274)
Gene Description:
coenzyme Q5, methyltransferase
Transcript:
RefSeq NM_032314.2 (NON-CURRENT)
Match location:
Position 663 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCGGAATGTCACACACAT
Hairpin Sequence:
5'-CCGG-GATCCGGAATGTCACACACAT-CTCGAG-ATGTGTGTGACATTCCGGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCGGAATGTCACACACATCTCGAGATGTGTGTGACATTCCGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCGGAATGTCACACACATCTCGAGATGTGTGTGACATTCCGGATC-3'

Other clones with same target sequence:

(none)