Construct: shRNA TRCN0000163380

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_197964.1-417s1c1
DNA Barcode:
GATGCTGCATTCATAGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FMC1 (154791)
Gene Description:
formation of mitochondrial complex V assembly factor 1 homolog
Transcript:
RefSeq NM_197964.1 (NON-CURRENT)
Match location:
Position 417 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163380)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCTGCATTCATAGGAGAA
Hairpin Sequence:
5'-CCGG-GATGCTGCATTCATAGGAGAA-CTCGAG-TTCTCCTATGAATGCAGCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCTGCATTCATAGGAGAACTCGAGTTCTCCTATGAATGCAGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCTGCATTCATAGGAGAACTCGAGTTCTCCTATGAATGCAGCATC-3'

Other clones with same target sequence:

(none)