Construct: shRNA TRCN0000163417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014008.1-1704s1c1
DNA Barcode:
GATGAGCTTGTGTTCAAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC22 (28952)
Gene Description:
coiled-coil domain containing 22
Transcript:
RefSeq NM_014008.1 (NON-CURRENT)
Match location:
Position 1704 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGCTTGTGTTCAAGGAT
Hairpin Sequence:
5'-CCGG-GATGAGCTTGTGTTCAAGGAT-CTCGAG-ATCCTTGAACACAAGCTCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGCTTGTGTTCAAGGATCTCGAGATCCTTGAACACAAGCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGCTTGTGTTCAAGGATCTCGAGATCCTTGAACACAAGCTCATC-3'

Other clones with same target sequence:

  1. TRCN0000292363