Construct: shRNA TRCN0000163439

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182523.1-399s1c1
DNA Barcode:
GAATACCTGAAGGAAAGGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMC1 (152100)
Gene Description:
C-X9-C motif containing 1
Transcript:
RefSeq NM_182523.1 (NON-CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163439)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATACCTGAAGGAAAGGGAA
Hairpin Sequence:
5'-CCGG-GAATACCTGAAGGAAAGGGAA-CTCGAG-TTCCCTTTCCTTCAGGTATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATACCTGAAGGAAAGGGAACTCGAGTTCCCTTTCCTTCAGGTATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATACCTGAAGGAAAGGGAACTCGAGTTCCCTTTCCTTCAGGTATTC-3'

Other clones with same target sequence:

(none)