Construct: shRNA TRCN0000163444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001008215.1-209s1c1
DNA Barcode:
GTTTGAAGGAAGGATACTGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COA5 (493753)
Gene Description:
cytochrome c oxidase assembly factor 5
Transcript:
RefSeq NM_001008215.1 (NON-CURRENT)
Match location:
Position 209 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGAAGGAAGGATACTGCA
Hairpin Sequence:
5'-CCGG-GTTTGAAGGAAGGATACTGCA-CTCGAG-TGCAGTATCCTTCCTTCAAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGAAGGAAGGATACTGCACTCGAGTGCAGTATCCTTCCTTCAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGAAGGAAGGATACTGCACTCGAGTGCAGTATCCTTCCTTCAAAC-3'

Other clones with same target sequence:

(none)