Construct: shRNA TRCN0000163642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017977.1-855s1c1
DNA Barcode:
GAAAGTGCTAAGTAACCTCGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRYBG2 (55057)
Gene Description:
crystallin beta-gamma domain containing 2
Transcript:
RefSeq NM_017977.1 (NON-CURRENT)
Match location:
Position 855 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGTGCTAAGTAACCTCGT
Hairpin Sequence:
5'-CCGG-GAAAGTGCTAAGTAACCTCGT-CTCGAG-ACGAGGTTACTTAGCACTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGTGCTAAGTAACCTCGTCTCGAGACGAGGTTACTTAGCACTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGTGCTAAGTAACCTCGTCTCGAGACGAGGTTACTTAGCACTTTC-3'

Other clones with same target sequence:

(none)