Construct: shRNA TRCN0000163644

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030573.1-344s1c1
DNA Barcode:
GCATCCGAGTACATCTACTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THAP7 (80764)
Gene Description:
THAP domain containing 7
Transcript:
RefSeq NM_030573.1 (NON-CURRENT)
Match location:
Position 344 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163644)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCGAGTACATCTACTTC
Hairpin Sequence:
5'-CCGG-GCATCCGAGTACATCTACTTC-CTCGAG-GAAGTAGATGTACTCGGATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCGAGTACATCTACTTCCTCGAGGAAGTAGATGTACTCGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCGAGTACATCTACTTCCTCGAGGAAGTAGATGTACTCGGATGC-3'

Other clones with same target sequence:

  1. TRCN0000319131