Construct: shRNA TRCN0000163657

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018271.2-1807s1c1
DNA Barcode:
GAAAGTTTCAGGGCCTGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THNSL2 (55258)
Gene Description:
threonine synthase like 2
Transcript:
RefSeq NM_018271.2 (NON-CURRENT)
Match location:
Position 1807 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163657)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGTTTCAGGGCCTGCAAA
Hairpin Sequence:
5'-CCGG-GAAAGTTTCAGGGCCTGCAAA-CTCGAG-TTTGCAGGCCCTGAAACTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGTTTCAGGGCCTGCAAACTCGAGTTTGCAGGCCCTGAAACTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGTTTCAGGGCCTGCAAACTCGAGTTTGCAGGCCCTGAAACTTTC-3'

Other clones with same target sequence:

(none)