Construct: shRNA TRCN0000163735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022896.1-1517s1c1
DNA Barcode:
GACCCAAACCTAGTGGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LPIN3 (64900)
Gene Description:
lipin 3
Transcript:
RefSeq NM_022896.1 (NON-CURRENT)
Match location:
Position 1517 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163735)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCCAAACCTAGTGGTGAAA
Hairpin Sequence:
5'-CCGG-GACCCAAACCTAGTGGTGAAA-CTCGAG-TTTCACCACTAGGTTTGGGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCCAAACCTAGTGGTGAAACTCGAGTTTCACCACTAGGTTTGGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCCAAACCTAGTGGTGAAACTCGAGTTTCACCACTAGGTTTGGGTC-3'

Other clones with same target sequence:

(none)