Construct: shRNA TRCN0000163781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017842.1-1175s1c1
DNA Barcode:
GCAACAAAGTCAAGAGGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC48A1 (55652)
Gene Description:
solute carrier family 48 member 1
Transcript:
RefSeq NM_017842.1 (NON-CURRENT)
Match location:
Position 1175 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACAAAGTCAAGAGGTCAA
Hairpin Sequence:
5'-CCGG-GCAACAAAGTCAAGAGGTCAA-CTCGAG-TTGACCTCTTGACTTTGTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACAAAGTCAAGAGGTCAACTCGAGTTGACCTCTTGACTTTGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACAAAGTCAAGAGGTCAACTCGAGTTGACCTCTTGACTTTGTTGC-3'

Other clones with same target sequence:

(none)