Construct: shRNA TRCN0000163842

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015686.1-614s1c1
DNA Barcode:
CCACTTTCGGAACTTCACTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM155B (27112)
Gene Description:
family with sequence similarity 155 member B
Transcript:
RefSeq NM_015686.1 (NON-CURRENT)
Match location:
Position 614 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163842)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTTCGGAACTTCACTCT
Hairpin Sequence:
5'-CCGG-CCACTTTCGGAACTTCACTCT-CTCGAG-AGAGTGAAGTTCCGAAAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTTCGGAACTTCACTCTCTCGAGAGAGTGAAGTTCCGAAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTTCGGAACTTCACTCTCTCGAGAGAGTGAAGTTCCGAAAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000278581