Construct: shRNA TRCN0000163903

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001371.1-1833s1c1
DNA Barcode:
CGGATAAGTGAGCCCATCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAH8 (1769)
Gene Description:
dynein axonemal heavy chain 8
Transcript:
RefSeq NM_001371.1 (NON-CURRENT)
Match location:
Position 1833 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163903)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATAAGTGAGCCCATCAAT
Hairpin Sequence:
5'-CCGG-CGGATAAGTGAGCCCATCAAT-CTCGAG-ATTGATGGGCTCACTTATCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATAAGTGAGCCCATCAATCTCGAGATTGATGGGCTCACTTATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATAAGTGAGCCCATCAATCTCGAGATTGATGGGCTCACTTATCCG-3'

Other clones with same target sequence:

(none)