Construct: shRNA TRCN0000163955

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022152.3-543s1c1
DNA Barcode:
CCGTTTCCCATGGAACATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMBIM1 (64114)
Gene Description:
transmembrane BAX inhibitor motif containing 1
Transcript:
RefSeq NM_022152.3 (NON-CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163955)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTTCCCATGGAACATCAT
Hairpin Sequence:
5'-CCGG-CCGTTTCCCATGGAACATCAT-CTCGAG-ATGATGTTCCATGGGAAACGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTTCCCATGGAACATCATCTCGAGATGATGTTCCATGGGAAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTTCCCATGGAACATCATCTCGAGATGATGTTCCATGGGAAACGG-3'

Other clones with same target sequence:

  1. TRCN0000292247