Construct: shRNA TRCN0000163964

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_197964.1-225s1c1
DNA Barcode:
CATGAGCTTCATTTCCAAGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FMC1 (154791)
Gene Description:
formation of mitochondrial complex V assembly factor 1 homolog
Transcript:
RefSeq NM_197964.1 (NON-CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000163964)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAGCTTCATTTCCAAGCT
Hairpin Sequence:
5'-CCGG-CATGAGCTTCATTTCCAAGCT-CTCGAG-AGCTTGGAAATGAAGCTCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAGCTTCATTTCCAAGCTCTCGAGAGCTTGGAAATGAAGCTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAGCTTCATTTCCAAGCTCTCGAGAGCTTGGAAATGAAGCTCATG-3'

Other clones with same target sequence:

  1. TRCN0000330660