Construct: shRNA TRCN0000164141

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152745.1-1952s1c1
DNA Barcode:
CCTGAAGACATGCTCTCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NXPH1 (30010)
Gene Description:
neurexophilin 1
Transcript:
RefSeq NM_152745.1 (NON-CURRENT)
Match location:
Position 1952 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164141)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGACATGCTCTCACAT
Hairpin Sequence:
5'-CCGG-CCTGAAGACATGCTCTCACAT-CTCGAG-ATGTGAGAGCATGTCTTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGACATGCTCTCACATCTCGAGATGTGAGAGCATGTCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGACATGCTCTCACATCTCGAGATGTGAGAGCATGTCTTCAGG-3'

Other clones with same target sequence:

(none)