Construct: shRNA TRCN0000164169

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052907.1-494s1c1
DNA Barcode:
CAGTTTGACATCACTGACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM132B (114795)
Gene Description:
transmembrane protein 132B
Transcript:
RefSeq NM_052907.1 (NON-CURRENT)
Match location:
Position 494 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164169)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTTGACATCACTGACCTT
Hairpin Sequence:
5'-CCGG-CAGTTTGACATCACTGACCTT-CTCGAG-AAGGTCAGTGATGTCAAACTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTTGACATCACTGACCTTCTCGAGAAGGTCAGTGATGTCAAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTTGACATCACTGACCTTCTCGAGAAGGTCAGTGATGTCAAACTG-3'

Other clones with same target sequence:

(none)