Construct: shRNA TRCN0000164240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015022.2-1591s1c1
DNA Barcode:
CGTCATCTCGATCATTGGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDZD2 (23037)
Gene Description:
PDZ domain containing 2
Transcript:
RefSeq NM_015022.2 (NON-CURRENT)
Match location:
Position 1591 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCATCTCGATCATTGGGTT
Hairpin Sequence:
5'-CCGG-CGTCATCTCGATCATTGGGTT-CTCGAG-AACCCAATGATCGAGATGACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCATCTCGATCATTGGGTTCTCGAGAACCCAATGATCGAGATGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCATCTCGATCATTGGGTTCTCGAGAACCCAATGATCGAGATGACG-3'

Other clones with same target sequence:

(none)