Construct: shRNA TRCN0000164264

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182512.1-525s1c1
DNA Barcode:
CTGCTACCATTTCCTTCCGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF534 (147658)
Gene Description:
zinc finger protein 534
Transcript:
RefSeq NM_182512.1 (NON-CURRENT)
Match location:
Position 525 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164264)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTACCATTTCCTTCCGTT
Hairpin Sequence:
5'-CCGG-CTGCTACCATTTCCTTCCGTT-CTCGAG-AACGGAAGGAAATGGTAGCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTACCATTTCCTTCCGTTCTCGAGAACGGAAGGAAATGGTAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTACCATTTCCTTCCGTTCTCGAGAACGGAAGGAAATGGTAGCAG-3'

Other clones with same target sequence:

(none)