Construct: shRNA TRCN0000164328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004943.1-502s1c1
DNA Barcode:
CAAGCCAATTGACAAGCGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DMWD (1762)
Gene Description:
DM1 locus, WD repeat containing
Transcript:
RefSeq NM_004943.1 (NON-CURRENT)
Match location:
Position 502 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCCAATTGACAAGCGGAT
Hairpin Sequence:
5'-CCGG-CAAGCCAATTGACAAGCGGAT-CTCGAG-ATCCGCTTGTCAATTGGCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCCAATTGACAAGCGGATCTCGAGATCCGCTTGTCAATTGGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCCAATTGACAAGCGGATCTCGAGATCCGCTTGTCAATTGGCTTG-3'

Other clones with same target sequence:

(none)