Construct: shRNA TRCN0000164381

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019067.3-1487s1c1
DNA Barcode:
CGGAGAATCTGATGAGCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNL3L (54552)
Gene Description:
G protein nucleolar 3 like
Transcript:
RefSeq NM_019067.3 (NON-CURRENT)
Match location:
Position 1487 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164381)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGAATCTGATGAGCTGTT
Hairpin Sequence:
5'-CCGG-CGGAGAATCTGATGAGCTGTT-CTCGAG-AACAGCTCATCAGATTCTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGAATCTGATGAGCTGTTCTCGAGAACAGCTCATCAGATTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGAATCTGATGAGCTGTTCTCGAGAACAGCTCATCAGATTCTCCG-3'

Other clones with same target sequence:

(none)